Here are JPG images of the Enzyme lab results, with some notes/comments [NOTE: your FA18 results are shown first, and then past semesters' results after, for comparison (if you want; I'll NOT ask you about past data -- only your own FA18)]

Exercises 1 & 2:

Referring back to your lab manual, what does Ex. 1 show us (below left)? Our controls (Tubes A1,B1,C1) showed that there was no contamination, so we can conclude that the enzyme Catalase does indeed facilitate the breakdown of H₂O₂ into H₂O and O₂ (released as a gas, and measured as mm of foam, about 29mm on average).

In Exercise 2 (below right), Tube B1 still served as a control (only substrate, H₂O₂, present, no enzyme). In B2, the presence of foam confirms that the enzyme is still present and active, even after already catalyzing the reaction in Exercise 1. So in an enzyme reaction, the enzyme is not 'used up', not destroyed, not changed, not broken down; it can do its job and then be used again (and again and...). Tube C1 showed an expected reaction, when you mixed enzyme with substrate already present from Ex. 1. And Tube C2 showed no reaction, indicating that there was NOT substrate left from Ex. 1; you had used up (broken down) all the substrate (reactant, H₂O₂) when you 'flicked' to maximum foam in Ex. 1.

Enzyme Lab (FA18) - Ex. 1&2.jpg

Enzyme Lab (FA18) - Ex. 1&2.jpg

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Past results (you do NOT need to deal with these; only here for my ongoing record)

SP18

Enzyme Lab (SP18) - Ex. 1&2 (7638).jpg

FA17:

Enzyme Lab (FA17) - Ex. 1&2.jpg

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Exercise 3 (effect of pH):

Here (FA18) we see an optimum pH of 7 (pH at which we measured maximal enzyme activity). And, of great importance, we see near-zero enzyme activity at pH 2 and 13, extremes of pH, very acidic and very basic. And after you returned these two vials back to optimum (pH 7), there was no increase in enzyme activity, suggesting that the low activity at extremes of pH was the result of denaturation of the enzyme (irreversible disruption of the 3-dimensional structure of the enzyme, including the active site).

FA18:

Enzyme Lab (FA18) - pH.jpg

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Past results (you do NOT need to deal with these; only here for my ongoing record)

SP18:

Enzyme Lab (SP18) - pH (7638).jpg

FA17:

Enzyme Lab (FA17) - pH.jpg

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Exercise 4 (Effect of Temperature):

FA18 results showed an optimum temperature for this enzyme of 37C, which was the only temperature used besides cold (frozen, 0C) and hot (boiling, 100C). Here we can say that (for FA18) the temperature optimum was about 37C, with zero activity at 0C and 100C. The high temperature treatment resulted in zero enzyme activity, which was NOT restored when we brought that tube back down to 37C (initial vs. final high temps), supporting the idea that the high temperature (boiling, in this case) denatured the enzyme, which was irreversible. The cold treatment for FA18 was frozen, so zero activity, but activity was restored when the vial was heated back up to 37C, showing NO denaturation from cold, just slowing down of the molecular movement.

Enzyme Lab (FA18) - Temp.jpg

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Past results (you do NOT need to deal with these; only here for my ongoing record)

SP18:

FA17:

Enzyme Lab (FA17) - Temp.jpg